Abstract
The simultaneous determination of ascorbic acid and uric acid has a major importance in biomedical and pharmaceutical science. A glassy carbon electrode modified with β-cyclodextrin entrapped in polyethyleneimine was developed for the determination of ascorbic and uric acids in binary mixtures using square wave voltammetry. In order to characterize the electrode modification, Raman spectra were obtained. The modified electrode was successfully applied for the determination of ascorbic and uric acid in urine with good recoveries. The limit of detection for ascorbic acid was 0.22 µM.
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