Simultaneous Determination of Amantadine and Rimantadine Residue in Animal Tissues by LC-MS/MS

Abstract

A rapid determination method for amantadine and rimantadine in animal tissues was established using liquid chromatography-electrospray tandem mass spectrometry(LC-ESI-MS/MS) in the multiple reaction monitoring(MRM) mode.The samples were extracted with acetonitrile-1.0% trichloroacetic acid(50∶50,V/V) solution in ultrasonic,followed by a cleanup procedure with mixed cation exchange SPE.The eluate was dried under nitrogen blow,then dissolved in 1 mL methanol-0.2% formic acid(10∶90,V/V) solution and the aliquot was detected by LC-MS/MS.The chromatographic retention time and mass fragment ion abundance ratio were taken for qualitative confirmation,and external standard method was employed for quantification.The limit of detection(LOD) is 0.4 μg/kg,and the limit of quantification(LOQ) is 1.0 μg/kg in chicken,chicken liver,pork and pork liver.The correlation coefficent is greater than 0.999(r20.999) of the four animal tissues are obtained within 0.5—100.0 μg/L linear ranges.Recoveries are in a range of 70.7%—92.3% with the relative standard deviations(RSDs) varied from 1.7% to 11.7% at spiked levels of 5.0—100μg/kg,respectively.