Abstract
A portable multiplex polymerase chain reaction (mPCR)-dual LFA system was developed for the simultaneous detection of P. carotovorum subsp. brasiliense and E. coli O157:H7 in fresh agricultural products. The field applicability of the system was improved using a portable PCR device and dual LFA strip, enabling rapid and sufficiently sensitive results to be obtained within 60 min. The mPCR-LFA method was validated for each bacterial pure culture and six agricultural products, with successful detection of both bacteria up to 102 CFU/g, a sufficiently sensitive level for ensuring the safety of all fresh agricultural products. The results demonstrate that the mPCR-LFA method developed in this study can identify pathogens much more conveniently in a point-of-care detection setting.
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