Abstract
SUMMARY The current postulate that organic acids added to a culture medium affect growth of fungi only by buffering the medium was tested by culturing Claviceps purpurea PRL 1980 in a medium containing glucose, ammonia, salts and 2, 3-14C-succinate and analyzing various fractions of the mycelium for the presence of radioactivity. In addition, radioactivity of respired C02 was measured. It was found that succinate was taken up before growth was evident and was assimilated and respired both before and after exogenous glucose was consumed. Radioactivity appeared in the hot water extract, ether-ethanol extract, hot TCA extract, protein extract, and in the crude cell wall preparation as well as in two sugars isolated from the cell wall. Thus, exogenous succinate participated in metabolism and did not influence the culture only in its capacity as a buffer.
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