Abstract
In this paper, a high-performance liquid chromatography with ultraviolet (HPLC-UV) detection method, using tetraazacalix[2]arene[2]triazine-modified silica gel (NCS) as solid-phase extraction (SPE) sorbent, was developed for extracting and purifying the two phytohormones (indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA)) in chili and wheat samples from different organs of chili and wheat. The limits of detectionwere about 0.02 and 0.04 μg/mL, and the limits of quantificationwere 0.04 and 0.20 μg/mL for IAA and IBA, respectively. The intraday and interday RSDs (n = 6) of peak areas and retention times were in the range of 0.76-1.20%. In addition, overall recoveries through the extraction and NCS-SPE purification ranged from 78.4% to 86.8% for IAA and IBA were obtained. Compared with the commercial SPE sorbents, NCS featured excellent selectivity to retain IAA and IBA in the sample matrices. In addition, the results more clearly indicated that highIAA and IBA content existed in roots and leaves of wheat and chili; in other organs of the plants, the concentration of the two phytohormones is lower. The results from theoretical computation were consistent with the retention behaviors of IAA and IBA on NCS. The proposedNCS-SPE-HPLC method is highly effective for trace analysis of the two phytohormones in plant samples.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call