Simulation of the effects of microplastics on the microbial community structure and nitrogen cycle of paddy soil

Abstract

Microplastics (MPs) are ubiquitous in farmland soils. However, few studies have evaluated their effects on the microbial community structure and nitrogen cycle of farmland soils. Here, 0.3% and 1% (mass percentage) of polyethylene terephthalate (PET), polyvinyl chloride (PVC), and polylactic acid (PLA) MPs were added to paddy soil to evaluate their impact on the paddy soil microenvironment. The alpha index of the PLA MP treatment was significantly different from that of the control group (p-value < 0.05). In contrast, the indices of the PET and PVC MP treatments were not different from the control (p-value > 0.05). Among the MP treatments, the alpha index of the PLA MP group was significantly different from the PET and PVC MP groups (p-value < 0.05). PCoA analysis also indicated that there were differences between PLA and other MP groups, and different MP concentrations and exposure times had a great impact on microbial composition. The three MPs affected NH4+ metabolism by changing the abundance of a NH2OH-forming gene (amoA) and an organic nitrogen-forming gene (gdh), as well as the abundances of Thiobacillus, Bradyrhizobium, Anaeromyxobacter, Geobacter, and Desulfobacca. Further, the MPs affected NO3− metabolism by regulating the abundance of the nirS and nirK genes and the abundance of Nitrospirae. In contrast, NO2− metabolism was not significantly affected by the MPs due to the low concentration of NO2−, which was attributed to the high abundance of nirS and nirK in the sample. Taken together, our findings indicated that MP addition may have an inhibitory effect on the nitrogen cycle in paddy soils and that the effect of degradable MPs may be greater than that of their non-degradable counterparts. Given the increasing severity of worldwide MP contamination, additional studies are required to assess their impact on global ecosystems and biogeochemical cycles.