Simulation of fermentolysis of secondary meat and bone raw materials

Abstract

Abstarct. The study is of current relevance due to an underestimated potential of secondary meat and bone raw materials, which contain valuable bioactive substances that can be used in food, feed, cosmetic and other products. Hydrolyzing of extracting protein, fat and mineral substances from such raw materials is a promising method. The study aimed to investigate and simulate fermentolysis of highly mineralized collagen-containing raw meat with proteolytic enzymes. The experiments were carried out on beef tubular and rib bones using Alcalase 2.5 L, Protamex and Protosubtilin G3x enzymes. The efficiency of protein hydrolysis was assessed with regard to the parameters of formol titratable nitrogen, dry matter, titratable acidity, which were determined in aqueous solution of the fermentolysat. The quality indicators of the hydrolysis products were assessed by standard physicochemical methods. The data obtained were processed by methods of mathematical statistics. A mathematical model of fermentolysis of meat and bone raw materials was developed based on the analysis of graphical dependences of formol-titrated nitrogen parameters and dry matter on the enzyme preparation dosage and process duration. The models can be used to assess the decomposition level of proteins and their water extraction for various types and dosages of enzyme preparations, and to determine rational duration of fermentolysis. The model can be used as a basis to reveal the effect of the main factors on the degree of hydrolysis of proteins in meat and bone raw materials and to control the process of fermentolysis.