Simulation of biochemical dynamics of [Formula: see text] and [Formula: see text] in fibroblast cell.

Abstract

Calcium dynamics is not only responsible for maintaining the framework and functions of the cell but also plays a role in the dynamics of other biochemical systems in the cell. Phospholipase C-[Formula: see text] l ([Formula: see text]) has a crucial role in the function of fibroblast cells. Experiments have shown that [Formula: see text] and [Formula: see text] have interdependent dynamics in fibroblast cells. However, no reaction-diffusion model exists for the two-way feedback system dynamics of [Formula: see text] and [Formula: see text] in fibroblasts till date. The computational model is designed to investigate the impact of variations in several processes, such as the [Formula: see text] pump, buffer process, source inflow, etc., on the system dynamics of [Formula: see text] and [Formula: see text] in fibroblast cells. The computational findings are obtained using finite element techniques, and the consequences of dysregulation in various processes on the spatiotemporal calcium and [Formula: see text] dynamics in fibroblasts are investigated. The results lead to the conclusion that the effects of buffer, source influx, diffusion, and [Formula: see text] pump can cause fluctuations in the dynamics of [Formula: see text] and [Formula: see text] in fibroblasts. Disruptions in these constitutive processes can result in changes in the dynamics of calcium and [Formula: see text]. Thus, the current model provides new/novel information regarding the precise dysregulatory constitutive systems that regulate calcium and [Formula: see text] kinetics, such as source inflow, diffusion, [Formula: see text], and buffer, can be responsible for excessive calcium and [Formula: see text] concentrations leading to fibrotic illnesses such as cancer and fibrosis.