SIMS depth profiling of polymer blends with protein based drugs

Abstract

We report the results of the surface and in-depth characterization of two component blend films of poly( l-lactic acid) (PLLA) and Pluronic surfactant [poly(ethylene oxide) (A) poly(propylene oxide) (B) ABA block copolymer]. These blend systems are of particular importance for protein drug delivery, where it is expected that the Pluronic surfactant will retain the activity of the protein drug and enhance the biocompatibility of the device. Angle dependant X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) employing an SF 5 + polyatomic primary ion source were both used for monitoring the surfactant's concentration as a function of depth. The results show an increased concentration of surfactant at the surface, where the surface segregation initially increases with increasing bulk concentration and then remains constant above 5% (w/w) Pluronic. This surface segregated region is immediately followed by a depletion region with a homogeneous mixture in the bulk of the film. These results suggest the selection of the surfactant bulk concentration of the thin film matrices for drugs/proteins delivery should achieve a relatively homogeneous distribution of stabilizer/protein in the PLLA matrix. Analysis of three component blends of PLLA, Pluronic and insulin are also investigated. In the three component blends, ToF-SIMS imaging shows the spatial distribution of surfactant/protein mixtures. These data are reported also as depth profiles.