Abstract
In this study, a simple, label-free, electrochemical immunosensor system, including a three-electrode transducer and a microchamber, was designed, fabricated, and integrated with focus toward the detection of Newcastle disease virus (NDV). The chicken egg yolk antibodies (IgY) against NDV were used as the biological recognition element, replacing purified IgG antibodies that require a complex extraction process and time-consuming. The IgY against NDV was immobilized on the sensor surface using PrA/GA and SAM/NHS approaches. The immunosensor showed high sensitivity with NDV concentrations ranging from 106 to 102 EID50/mL with good specificity, repeatability, and small standard deviations. Compared to traditional methods, the immunosensor with advantages such as simple fabrication, quick response, direct detection, and possibility for miniaturization by integrating the immunosensor with the microchamber is potential for applications in contamination studies and field measurements.
Highlights
Newcastle disease (ND) is one of the most contagious diseases in poultry that has widely spread in South East Asian countries including Vietnam and causes severe economic losses [1, 2]
The three-electrode setup including the integrated Au electrodes consisted of a working electrode (WE), a counter electrode (CE) in a chip, and a quasireference Ag/AgCl electrode was designed and fabricated
The measured peak current (Ianodic and Icathodic) and the difference between the anodic and cathodic peak potentials, ΔE, stayed almost the same after 20 cyclic voltammetry (CV) cycles
Summary
Newcastle disease (ND) is one of the most contagious diseases in poultry that has widely spread in South East Asian countries including Vietnam and causes severe economic losses [1, 2]. Label-free electrochemical immunosensor detects antigens using specific antibodies immobilized on a surface of metallic electrodes [8,9,10,11] This method has been developed with rapid analysis, high sensitivity, and portability purposes [12,13,14,15]. The polydimethylsiloxane- (PDMS-) based microchamber was bonded with the integrated three-electrode setup to facilitate miniaturization of the analytical system, and the volume of the consumed biological sample is reduced.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
