Abstract
In this study, a label-free electrochemical immunosensor system was designed and fabricated with focus on a detection of Newcastle disease virus (NDV). The chicken egg yolk antibodies (IgY) against NDV was used as the biological recognition element to replace IgG antibody, which offers some advantages with respect to animal care, high productivity and special suitability in the source of antibodies. The IgY against NDV was immobilized on sensor surface using the chemical approach. The immunosensor showed high sensitivity with NDV concentration range from 106 to 102 EID50/mL. This relatively simple fabrication is potential for applications in infection studies.
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