Abstract
A simple high-performance liquid chromatographic method using UV detection was developed for the determination of α-tocopherol in human plasma. The method entailed direct injection of the plasma sample after deproteinization using acetonitrile–tetrahydrofuran (3:2). The mobile phase comprised methanol–tetrahydrofuran (94:6) and analysis was run at a flow-rate of 1.5 ml/min with the detector operating at 292 nm. A Crestpak C18S (5 μm, 250 mm×4.6 mm ID) was used for the chromatographic separation. The method had a mean recovery of 93%, while the within-day and between-day coefficients of variation and percentage errors were all less than 7%. The speed, specificity, sensitivity and reproducibility of this method make it particularly suitable for routine determination of α-tocopherol in human plasma. Moreover, only a small sample plasma volume (100 μl) is required for the analysis.
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