Abstract
Simple graphical plots in distribution-free space are shown to provide estimates of mechanism and kinetic parameters for complex steady-state processes. The methods were tested using simulated 1:1, 1:2, 2:2, 2:3 and 3:3 functions, and real data from polyol dehydrogenase, NADPH oxidation in the presence of DL-glyceraldehyde, and NADPH oxidation in the presence of an NADPH-binding protein. Estimates of mechanism and parameters agreed closely with those found from non-linear regression by computer.
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