Simple, fast and accurate method for the determination of glycogen in the model unicellular cyanobacterium Synechocystis sp. PCC 6803

Abstract

Glycogen is a highly soluble branched polymer composed of glucose monomers linked by glycosidic bonds that represents, together with starch, one of the main energy storage compounds in living organisms. While starch is present in plant cells, glycogen is present in bacteria, protozoa, fungi and animal cells. Due to its essential function, it has been the subject of intense research for almost two centuries. Different procedures for the isolation and quantification of glycogen, according to the origin of the sample and/or the purpose of the study, have been reported in the literature. The objective of this study is to optimize the methodology for the determination of glycogen in cyanobacteria, as the interest in cyanobacterial glycogen has increased in recent years due to the biotechnological application of these microorganisms. In the present work, the methodology reported for the quantification of glycogen in cyanobacteria has been reviewed and an extensive empirical analysis has been performed showing how this methodology can be optimized significantly to reduce time and improve reliability and reproducibility. Based on these results, a simple and fast protocol for quantification of glycogen in the model unicellular cyanobacterium Synechocystis sp. PCC 6803 is presented, which could also be successfully adapted to other cyanobacteria.