Abstract
Herein, we describe the construction of a simple, low-cost, and fully disposable microfluidic device (DμFD) for ultrasensitive Salmonella typhimurium (S. typhi) detection. The DμFD is based on unmodified carbon electrode array for rapid detection of S. typhi in milk samples by magneto-immunoassay using gold nanoparticles (AuNPs) as a label. In the method proposed, S. typhi was magnetically captured and separated from samples using magnetic beads modified with a monoclonal anti-Salmonella antibody followed by the addition of polyclonal anti-Salmonella antibody labeling with AuNPs. The magneto-immunoconjugate formed was injected into the DμFD and captured on the electrode surface by placing magnets behind the unmodified working electrodes. The S. typhi detection was carried out by the electrochemical response of the gold from AuNPs. A single DμFD was used for rapid and full S. typhi analysis by measuring standards and samples simultaneously. A low limit of detection (LOD) of 7.7cellsmL−1 for S. typhi in a linear range from 10.0 to 100.0cellsmL−1 was obtained. The DμFD proved to be fast, accurate, and inexpensive for S. typhi detection and has great potential to multiplex detection of different food contaminants.
Published Version
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