Abstract
This chapter discusses some simple considerations on the design of preparative columns. The design of the column is a very important aspect in preparative liquid chromatography (PLC). The decrease in column efficiency with increasing injected quantity is an important matter in PLC since a certain number of plates is required to obtain a given purity. Small particles are associated with shorter and bulkier columns than large particles, while at the same time giving less back pressure. Mass overload is a thermodynamic effect related to the nonlinearity of the distribution isotherm. Concentration overload is a more complex situation, and no accurate mathematical solution is yet available to describe the peak profiles. Mass overload is a very important problem in PLC because it is usually associated with much larger throughputs than volume overload.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
