Abstract

Abstract Episulfides were determined by a simple colorimetric assay using 4-(p-nitrobenzyl)-pyridine, which has been used previously for the detection and analysis of epoxides, organophosphates, and other alkylating agents. With episulfides, absorbance was directly proportional to concentration up to an absorbance of at least 1.0. No interference was observed with a variety of non-episulfide sulfur-containing substances. The colorimetric method is useful in monitoring 1-cyanoepithioalkane formation from the degradation of glucosinolates containing terminal double bonds.

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