Abstract
ABSTRACTDetermining seed phytate concentration in soybeans [Glycine max (L.) Merr.] is important for screening germplasm and for selection of modified‐phosphorus (P) lines with stable low‐phytate expression. Accurate quantitative phytate assays and simple colorimetric assays have been developed; however, a high‐throughput colorimetric phytate assay is needed. The objectives of this study were to develop a cost‐ and time‐effective modified Fe colorimetric phytate assay, and to demonstrate that the assay can be used for direct selection of phytate concentration in soybean seeds. The new modified Fe colorimetric assay was tested on 192 samples from eight lines from three germplasm sources (normal‐P composition, and two different modified‐P composition mutants) produced in 12 environments. Compared to other quantitative and colorimetric methods of phytate determination, this assay is faster, less expensive, and simpler, which meets the needs of breeders for selection among modified‐P soybean lines over environments.
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