Abstract
The separation of 21 free amino acids as their isobutyl N(O,S)-pentafluorobenzoyl derivatives by capillary gas chromatography with electron-capture detection is described. This method has been successfully applied to routine analysis of most of these amino acids in brain tissue and urine. The amino acids are converted to their isobutyl N(O,S)-penta-fluorobenzoyl derivatives by acid-catalysed esterification followed by aqueous extractive pentafluorobenzoylation. The 21 amino acids can be separated on a single capillary column in 21 min. The high sensitivity permits analysis in minute quantities of sample and the high stability permits the use of an automatic sample injector at ambient temperatures. Thus, over 50 samples can be readily analyzed in a single day.
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Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
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