Abstract
A direct, extraction-free spectrophotometric method has been developed for the determination of acebutolol hydrochloride (ABH) in pharmaceutical preparations. The method is based on ionpair complex formation between the drug and two acidic dyes (sulphonaphthalein) namely bromocresol green (BCG) and bromothymol blue (BTB). Conformity to Beer’s law enabled the assay of the drug in the range of 0.5-13.8 μg mL-1 with BCG and 1.8-15.9 μg mL-1 with BTB. Compared with a reference method, the results obtained were of equal accuracy and precision. In addition, these methods were also found to be specific for the analysis of acebutolol hydrochloride in the presence of excipients, which are co-formulated in the drug.
Highlights
Acebutolol hydrochloride (Fig 1) is (N-[3-Acetyl-4-[2-hydroxy-3[(1-methylethyl)amino]propoxy]phenyl]butanamide) hydrochloride, is a cardio-selective betablocker used in the management of hypertension, angina pectoris and cardiac arrhythmias[1], which normalizes the blood pressure and prevents the occurrence of hypertensive crisis
We describe the application of acidic dyes to the spectrophotometric determination of acebutolol hydrochloride (ABH) without the application of buffers
Absorption spectra The structure of ABH features its basic nature. This structure suggests the possibility of utilizing an anionic dye as chromogenic reagent
Summary
Acebutolol hydrochloride (Fig 1) is (N-[3-Acetyl-4-[2-hydroxy-3[(1-methylethyl)amino]propoxy]phenyl]butanamide) hydrochloride, is a cardio-selective betablocker used in the management of hypertension, angina pectoris and cardiac arrhythmias[1], which normalizes the blood pressure and prevents the occurrence of hypertensive crisis. Sungur and Yurdakul[7] have determined acebutolol by UV Spectrophotometry When compared to this method, our method is simple and does not involve any tedious/complex reaction conditions. This prompted us to develop simple, sensitive and accurate spectrophotometric methods for the determination of ABH in pure and pharmaceutical formulations. One of the well-established spectrophotometric methods is through ion-pair complex extraction. In this case, an ion-pair is formed between a basic compound and an anionic dye (e.g., bromophenol blue, bromocresol green, Ecl. Quím., São Paulo, 33(2): 37-40, 2008 methyl orange, etc.). Solving 10 mg in 100 mL of chloroform and further diluted as and when required
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