Abstract
Feed rate of multiple substrates containing glucose, polypeptone and yeast extract was readily optimized for a high density cell culture using a multiple perfusion culture system. Three independent perfusion cultures of Lactococcus lactis were carried out simultaneously using shaken ceramic membrane flasks. Stepwise control of substrate feed rate was found to be the most effective in enhancing the cell growth and in reducing both the dilution rate and the total volume of the substrate solution. This optimized control strategy was applied to a scale-up culture system that employed a stirred ceramic membrane reactor.
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