Abstract
Oxygen equilibria of hemoglobin in the presence of different concentrations of 2,3-diphosphoglycerate were simulated by using Adair's stepwise oxygenation scheme in which changes of the concentration of free 2,3-diphosphoglycerate during oxygenation were taken into account. The calculated equilibrium curves fitted well to experimental points and also well expressed biphasic features in the presence of less than 1 mole of the phosphate per mole of hemoglobin.
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