Abstract
This chapter presents measurement of accurate oxygen equilibrium curves by an automatic oxygenation apparatus. The methods for measuring the oxygen equilibrium curve of hemoglobin are roughly separated into two groups: the static method and the dynamic method. In the static method, the curve is measured on a point-by-point base: it is obtained as a set of a finite number of points that are determined when the equilibrium between hemoglobin and oxygen has been established. The chapter discusses an automatic oxygenation apparatus and related techniques. This apparatus was originally designed to provide an easy and rapid means for studying the functional properties of increasing number of abnormal hemoglobins. The apparatus was further improved to measure more accurate equilibrium curves of more concentrated hemoglobin solutions. This improved method enables the measurement of equilibrium curves, which accurately allow the evaluation of the four oxygen equilibrium constants or the parameters of allosteric models by a least squares method.
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