Simian immunodeficiency virus from mandrill ( Mandrillus sphinx) SIVmnd experimentally infects human and nonhuman primate cells

Abstract

This study set out to characterize the features of experimental infection by simian immunodeficiency virus in mandrill (SIVmnd) ( Mandrillus sphinx), cynomolgus macaque ( Macaca fascicularis), rhesus macaque ( Macaca mulatta), chimpanzee ( Pan troglodytes), African green monkey ( Cercopithecus pygerythrus), baboon ( Papio cynocephalus) and human cells. Purified cells were exposed to a primary isolate of SIVmnd grown in the infected mandrill peripheral blood mononuclear cells, and viral p27 gag antigen was quantitated by antigen capture ELISA. Human cells have been found to be infected by SIVmnd. SIVmnd infection in cynomolgus macaque, rhesus macaque, baboon, mandrill and human cells were more effective than in vervet and chimpanzee cells. In addition, the lymphocytic cell lines SupT1, CEMx174 and Molt4 clone 8 were consistently infected by SIVmnd, whereas U937, a monocytic cell line, was not.