Abstract
An H3/H4 histone chaperone, Asf1, plays an essential role in maintaining genomic stability in many species, including fission yeast. Here, we showed that overexpression of a CENP-A chaperone Sim3 suppressed the temperature sensitive phenotype of asf1–33 and asf1–30 mutants and the defect in chromatin structure, and prevented the accumulation of DNA damage in asf1–33 mutants at high temperatures. Furthermore, asf1–33 and Δsim3 were synthetic lethal. Consistent with this, shutdown of sim3 expression in asf1–33 Δsim3 double mutants that contained extragenic sim3 resulted in growth retardation. In addition, the Δsim3 mutant displayed sensitivity to thiabendazol and hydroxyurea, which suggests that Sim3 plays a general role in maintaining chromatin structure. Our results suggest a possibility that Sim3 functions as a histone chaperone.
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