Abstract
ABSTRACT Rapid non-laboratory screening of plants for pathogenic viruses crucially influences crop yields in modern agricultural technologies. The aim of this study was to develop a highly-sensitive lateral flow immunoassay (LFIA) for rapid detection of potato leafroll virus (PLRV), an infectious agent of one of the most widespread potato diseases. The proposed LFIA combines the formation of sandwich immune complexes with gold nanoparticles (GNP) as labels and silver enhancement. The enhancement stage was realized using mixture of silver lactate and hydroquinone and subsequent addition of chloride-containing buffer to stop silver reduction. LFIA with silver enhancement was 15 times more sensitive (detection limit 0.2 ng/mL; 15 min) compared with conventional LFIA (detection limit 3 ng/mL; 10 min). The enhanced LFIA detected PLRV in leaves’ extracts of infected potato in dilutions higher than enzyme-linked immunosorbent assay.
Highlights
Viral and bacterial infections are among the main causes of crop yield loss (Narayanasamy, 2011a, 2011b; Sanfacon, 2017)
Key advantages of the lateral flow immunoassay (LFIA) are short analysis time (10–15 min), easy sample preparation, and the fact that there is no requirement for expensive stationary equipment
We have proposed the use of silver enhancement to detect low concentrations of potato leafroll virus (PLRV) by LFIA
Summary
Viral and bacterial infections are among the main causes of crop yield loss (Narayanasamy, 2011a, 2011b; Sanfacon, 2017). One of the most suitable methods for addressing this need is lateral flow immunoassay (LFIA), called immunochromatographic assay. Key advantages of the LFIA are short analysis time (10–15 min), easy sample preparation, and the fact that there is no requirement for expensive stationary equipment. LFIA is currently one of the leading techniques for routine detection of various compounds (Bahadir & Sezginturk, 2016; Dzantiev, Byzova, Urusov, & Zherdev, 2014; Quesada-Gonzalez & Merkoci, 2015). LFIA is a priority method for on-site detection of pathogens (Danks & Barker, 2000; Jones, 2014; Khater, de la Escosura-Muniz, & Merkoci, 2017). LFIAs are included in functioning national and international regulations for agricultural control (EPPO, 2014)
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