Abstract
BackgroundBurn wounds are one of the causes of cutaneous injury that involve both epidermal and dermal layers of skin. Silver sulfadiazine (SSD) has been widely used to treat burn wounds, however recent studies have found the treatment to have some drawbacks, such as cellular toxicity effects. Cutaneous wound regeneration is known to start from the basal layer of the epidermal epithelial cells, which are enriched with highly proliferative cells. Keratin-19 (K19) is one of the epidermal stem cell biomarkers found in the skin. This study aims to explore the expression of K19 in burn wound tissue and to investigate the effect of SSD on its expression.MethodsWe created a burn wound model in Sprague Dawley rats and randomly divided them into control and SSD groups. Wound closure was evaluated (visitrak) overtime series followed by histological evaluation of K19 expression in the wound tissue (immunohistochemistry staining).ResultsOur model successfully represents full-thickness damage caused by a burn wound. The SSD group showed a faster reduction of wound surface area (wound closure) compared to the control group with the peak at day 18 post wounding (p < 0.05). K19 expression was found in both groups and was distributed on epidermal layers, hair follicles and dermis of granulation tissue showing similar patterns.ConclusionTopical application of SSD on burn wounds showed superiority in wound closure and is likely to have no harmful effect on epidermal stem cells. However, further study is required to investigate the effect of silver species on cell viability and toxicity effects during long term treatment.
Highlights
Burn wounds can be devastating due to their longer healing period, which is often followed by incomplete healing with scar formation and functional or esthetic impairment [1e 3]
We aim to investigate the effect of topical Silver sulfadiazine (SSD) on burn wound healing and its effect on the expression of K19 in wound tissue
Our results showed that the SSD group presented smaller wound surface areas than control group starting at day 6 and reaching a peak at day 18 ( p < 0.05), completely closing at day 24 post-wounding (Fig. 2)
Summary
Burn wounds can be devastating due to their longer healing period, which is often followed by incomplete healing with scar formation and functional or esthetic impairment [1e 3]. The most common treatment for burn wounds is silver sulfadiazine (SSD), which has been used for decades for its antimicrobial activity. The availability of silver ions is dependent upon dissociation of silver salts or on their solubility in wound fluids, such as wound exudate This ionic silver will interact with functional organic groups such as thiols, which act as the key components of prokaryotic cell wall structures and nucleic acids (DNA) which results in membrane porations and causes cell damage or a reduced ability to proliferate [4e7]. This interaction is the basic antimicrobial mechanism of SSD. This study aims to explore the expression of K19 in burn wound tissue and to investigate the effect of SSD on its expression
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
