Silk protein as a cell additive

Abstract

Event Abstract Back to Event Silk protein as a cell additive You-Young Jo1, Haeyong Kweon1 and Kwang-Gill Lee1 1 National Academy of Agricultureal Science, Department of Agricultural BIology, Korea Introduction: A substance for the cell proliferation, additives such as cell cytokine is a very expensive. It is known that silk protein supports effectively cell proliferation[1]-[4]. Although there are many varieties of Bombyx mori silkworm, the effect of silkworm varieties on cell proliferation has not been considered in detail. To develop the cell additives using silk proteins, we studied that effects on cell proliferation and characteristics of silk obtained from Korea various silkworm varieties. Materials and Methods: Silk sericin was prepared under high temperature high pressure condition. Silk fibroin was prepared using CaCl2 : H2O : ethanol (1:8:2) with different dissolution time(1, 3, 5 hour). The characteristics of each silk protein were analyzed by performing Tensile strength, amino acid composition, porosity, XRD and gel electrophoresis. We investigated the attachment, proliferation, morphology of the cells and the expression levels of genes related to cell growth. Results: There are differences in silk cocoon from different silkworm varieties. The porosity of cocoons was 73~85%. The crystal structure of each cocoon was different by XRD analysis[5]. Also amino acid composition was different. But the molecular weight of silk proteins was same. The proliferation was accelerated in the presence all of silk sericin and silk fibroin. Especially, this was the best Baekokjam silk sericin in cell proliferation. The Baekokjam silk serin was upregulated the EGF gene expression up to 5 times. Conclusion: We expect that silk proteins could be a preferable culture medium supplement for stimulating the proliferation of cell. Then, these results suggest silk as a new material for medium supplement. This study was carried out with the support of "Research Program for Agricultural Science & Technology Development (Project No. PJ010006)", National Academy of Agricultural Science, Rural Development Administration, Republic of Korea