Abstract
ABSTRACT It is known that silk protein supports effectively proliferation of cell such as insect cell and hybridoma cell. Althoughthere are many varieties of Bombyx mori silkworm, the effect of silkworm varieties on cell proliferation has not beenconsidered in detail. We studied that characteristics of silk cocoon obtained from Baegokjam, Kumokjam, Daeseongjamsilkworm varieties and whether silk protein affected cell proliferation or not. Silk sericin was prepared under high tem-perature and high pressure condition. Silk fibroin was prepared using CaCl 2 :H 2 O : EtOH with different dissolutiontime. As a result, there are differences in silk cocoon from different silkworm varieties about cell proliferation. The pro-liferation was accelerated in the presence of Baegokjam silk sericin and Kumokjam silk fibroin with 5hr dissolutiontime. We expect that silk proteins could be a preferable culture medium supplement for stimulating the proliferation ofcell. Then, this results suggest silk as a new material for medium supplement replacing with fetal bovine serum.Key words : Silk fibroin, Silk sericin, Silkworm variety, Cell proliferation
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