Abstract

A method is presented for fabricating supported phospholipids bilayer on silicon surface for biosensor based on imaging ellipsometry. The lipid bilayer prepared by covalent immobilization of phosphatidylethanolamine layer on silicon surface followed by adsorption of PEG functionalized phospholipids layer, is highly protein resistant and stable, which can withstand multiple cycles of drying and rehydration, remaining intact in various buffers. Proteins covalently immobilized on the bilayer by the terminal p-nitrophenylcarbonyl or carboxyl group of PEG remain higher bioactivity than that immobilized on silicon surface directly.

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