Abstract

Application of supported phospholipids bilayers (SPB) is presented for biosensor based on imaging ellipsometry. One SPB is prepared by covalent immobilization of phospholipids monolayer on silicon surface, and followed by adsorption of PEG functionalized phospholipids layer. Another is prepared by adsorption of the mixture of phospholipids and PEG functionalized phospholipids on the hydrophilic silicon surface. Results show that obvious resistance to non-specific protein adsorption on SPB is obtained, and proteins covalently immobilized on the bilayers by the terminal carboxyl group of PEG remain higher bioactivity than that immobilized on silicon surface directly.

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