Silicon assisted ameliorative effects of iron nanoparticles against cadmium stress: Attaining new equilibrium among physiochemical parameters, antioxidative machinery, and osmoregulators of Phaseolus lunatus

Abstract

Currently, producing safe agricultural commodities from the crop plants cultivated in the soil with increasing heavy metal toxicity is a gigantic challenge in front of researchers. Heavy metals are absorbed and translocated in the crop plants and then transferred to every downstream consumer of the food chain, including humans, causing serious disorders and ailments. The current research presents a combined schematic application of iron nanoparticles (Fe-NPs) and/or silicon (Si), to mitigate cadmium (Cd) stress in Lima bean (Phaseolus lunatus). It was noted that Cd-induced toxicity curtailed growth, antioxidative machinery, glyoxalase system and nutrient uptake of the plants. Furthermore, the physiochemical features of Cd stressed plants, including carotenoids, chlorophyll, photochemical quenching, photosynthetic efficiency, and leaf relative water contents, were improved by the combined application of Si and Fe-NPs. Moreover, higher levels of malondialdehyde (MDA), methylglyoxal (MG), hydrogen peroxide (H2O2), and electrolyte leakage (EL) were observed in Cd stressed plants. Nevertheless, the independent treatment or combined application of Si and/or Fe-NPs attenuated the adversative effects of Cd on the aforementioned growth attributes. Furthermore, Si and Fe-NPs defended plants from the injurious effects of MG by improving the activities of the glyoxalase enzyme. The Si and Fe-NPs reduced Cd contents but at the same time improved uptake and accumulation of nutrients in treated plants exposed to the Cd regime. This study highlights that Si and Fe-NPs have enormous potential to mitigate Cd-induced phytotoxicity by declining Cd uptake and improving the growth attributes of plants if applied in combination.