Modulation of growth and key physiobiochemical attributes after foliar application of zinc sulphate (ZnSO4) on wheat (Triticum aestivum L.) under cadmium (Cd) stress.

Abstract

A pot experiment was conducted to examine the effect of foliar application of various levels of ZnSO4 on wheat (Triticum aestivum L.) under cadmium (Cd) stress. Seeds of two wheat varieties i.e., Ujala-2016 and Anaj-2017 were sown in sand filled plastic pots. Cadmium (CdCl2) stress i.e., 0 and 0.5mM CdCl2 was applied in full strength Hoagland's nutrient solution after 4weeks of seed germination. Foliar spray of varying ZnSO4 levels i.e., 0, 2, 4, 6 and 8mM was applied after 2weeks of CdCl2 stress induction (of 6week old plants). After 3weeks of foliar treatment leaf samples of 9week old wheat plants were collected for the determination of changes in various growth and physiobiochemical attributes. Results obtained showed that cadmium stress (0.5mM CdCl2) significantly decreased shoot and root fresh and dry weights, shoot and root lengths, yield attributes, chlorophyll a contents and total phenolics, while increased hydrogen peroxide (H2O2), total soluble proteins, free proline, glycinebetaine (GB) contents, and activities of antioxidant enzymes i.e., catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POD). Foliar application of varying ZnSO4 levels significantly increased various growth attributes, chlorophyll b contents, H2O2, free proline, GB and activities of antioxidant enzymes i.e., CAT, POD and APX, while decreased relative water contents and total phenolics under Cd stress or non stress conditions. Furthermore, both wheat varieties showed differential response under Cd stress and towards foliar application of ZnSO4 e.g., wheat var. Ujala-2016 was higher in shoot dry weight, root length, root fresh and dry weights, total leaf area per plant, 100 grains weight, number of tillers per plant, chlorophyll b, hydrogen peroxide (H2O2), activities of APX, POD, glycinebetaine and leaf free proline contents, while var. Anaj-2017 exhibited high shoot fresh weight, grain yield per plant, no. of grains per plant, chlorophyll contents, chlorophyll a/b ratio, total phenolics, MDA and total soluble protein contents under cadmium stress or non stress conditions.