Abstract
Quorum sensing is a cell-to-cell communication system in bacteria that controls the production of virulence factors. Serratia marcescens is a causative agent of hospital-acquired infections that shows high resistance to antibiotics. This makes the treatment of these infections difficult. Quorum sensing regulates the production of virulence factors of S. marcescens such as prodigiosin, protease, swimming and swarming motilities and formation of biofilms. Inhibition of quorum sensing may be an alternative to antibiotic treatment to avoid emergence of resistance. Testing the ability of glyceryl trinitrate to inhibit quorum sensing and virulence factors of Serratia marcescens. The inhibiting activities of sub-inhibitory concentration of glyceryl trinitrate against the quorum-sensing regulated violacein pigment in Chromobacterium violaceum CV026 was performed to evaluate the anti-quorum sensing effect of glyceryl trinitrate. The anti-virulence activity was assessed against prodigiosin, protease, biofilm formation in addition to swimming and swarming motilities. Glyceryl trinitrate at at a concentration of 0.25 mg/ml produced significant inhibitory effects against violacein (67.01%), prodigiosin (82.67%), protease, swimming (36.72%) and swarming (79.31%) motilities and biofilm formation (87.83%). Glyceryl trinitrate is a quorum sensing and virulence inhibitor that may be useful in treatment of nosocomial infections caused by Serratia marcescens.
Highlights
Serratia marcescens is a nosocomial pathogen that can lead to many nosocomial infections such as those affecting urinary tract, respiratory tract in addition to wound in- African@ 2018 Abbas et al; licensee African Health Sciences
This study aimed to investigate the potential anti-quorum sensing and anti-virulence activities of Glyceryl trinitrate (GTN) against the nosocomial pathogen Serratia marcescens
Determination of Minimum Inhibitory Concentration (MIC) Glyceryl trinitrate could not inhibit the growth of Serratia marcescens isolate at 0.5 mg/ml which is the maximum available concentration, so MIC is equal to or higher than 1 mg/ml. the concentration selected to test the anti-quorum sensing and anti-virulence activities of GTN is 0.25 mg/ml which is equivalent to 1⁄4 MIC or less
Summary
Serratia marcescens is a nosocomial pathogen that can lead to many nosocomial infections such as those affecting urinary tract, respiratory tract in addition to wound in- African@ 2018 Abbas et al; licensee African Health Sciences. That quorum sensing inhibition is one useful approach that can help in treating infections without exerting stress on the growth of bacteria to avoid the evolution of antibiotic resistance. It can enhance the immune clearance of the pathogens[7,8]. This study aimed to investigate the potential anti-quorum sensing and anti-virulence activities of GTN against the nosocomial pathogen Serratia marcescens. Quorum sensing regulates the production of virulence factors of S. marcescens such as prodigiosin, protease, swimming and swarming motilities and formation of biofilms. Conclusion: Glyceryl trinitrate is a quorum sensing and virulence inhibitor that may be useful in treatment of nosocomial infections caused by Serratia marcescens.
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