Silencing SlGID2, a putative F-box protein gene, generates a dwarf plant and dark-green leaves in tomato

Abstract

In plant, F-box protein participates in various signal transduction systems and plays an important role in signaling pathways. Here, a putative F-box protein, namely SlGID2, was isolated from tomato (Solanum lycopersicum). Bioinformatics analyses suggested that SlGID2 shows high identity with F-box proteins from other plant species. Expression pattern analysis showed that SlGID2 gene is ubiquitously expressed in tomato tissues. To study the function of SlGID2 in tomato, SlGID2-silenced (SlGID2i) tomato by RNA interference (RNAi) was generated and displayed a dwarf plant and dark-green leaf phenotypes. The defective stem elongation of SlGID2i lines was not rescued by exogenous GA and its endogenous GA level was higher than wild type, further supporting the observation that SlGID2i transgenic plants are GA insensitive. Furthermore, SlGAST1, the downstream gene of GA signaling, and some cell expansion, division related genes (SlCycB1;1, SlCycD2;1, SlCycA3;1, SlXTH2, SlEXP2, SlKRP4) were down-regulated by SlGID2 silencing. In addition, the expression levels of SlDELLA (a negative regulator of GA signaling) and SlGA2ox1 were decreased, while SlGA3ox1 and SlGA20ox2 transcripts were increased in SlGID2i lines. Thus, we conclude that SlGID2 may be a positive regulator of GA signaling and promotes the GA signal pathway.