Abstract
Objective To evaluate the effect of ANO9 on the radiosensitivity of pancreatic cancer cell AsPC-1, aiming to provide new targets for clinical radiotherapy of pancreatic cancer. Methods Western blot was performed to detect the expression of ANO9 in pancreatic cancer cell lines (BxPC-3, PANC-1, AsPC-1) and normal pancreatic cell line (HPNE). The AsPC-1 cell line with stable silencing ANO9 was constructed by using lentivirus and validated by Western blot. MTT assay was adopted to detect the cell viability of AsPC-1 with stable silencing ANO9 after irradiation. Colony formation assay was conducted to evaluate the effect of silencing ANO9 upon the radiosensitivity of AsPC-1 cells. Western blot was performed to assess the effect of ANO9 silencing on the expression of EGFR/ERK signaling protein. Results The expression levels of ANO9 were significantly up-regulated in three pancreatic cancer cell lines compared with that in the normal pancreatic cell line HPNE (t=7.426, 5.543, 11.850, all P<0.05). After silencing ANO9, the expression level of ANO9 protein was significantly down-regulated than that in the control group (t= 9.670, P<0. 05). The AsPC-1 cells with stable silencing ANO9 were successfully constructed. The sensitivity of AsPC-1 cells to irradiation was significantly increased after silencing ANO9, and the sensitivity enhancement ratio was 1.566. The expression levels of EGFR/ERK signaling proteins (EGFR and p-ERK1/2) were significantly down-regulated after silencing ANO9(t=7.949, 13.160, both P<0.05). Conclusions Silencing ANO9 can significantly increase the sensitivity of AsPC-1 cells to radiotherapy, which is probably associated with the inhibition of EGFR/ERK signaling transduction. ANO9 might be a new therapeutic target for preventing the progression of pancreatic cancer. Key words: ANO9 gene; Pancreatic cancer cell line; Radiosensitivity
Published Version
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