Sidik Jari DNA 88 Plasma Nutfah Ubi Jalar di Indonesia Berdasarkan Delapan Penanda SSR

Abstract

<p>DNA Fingerprinting of Indonesian 88 Sweet Potato<br />Germplasm Based on Eight SSR Markers. Nurul<br />Hidayatun, Chaerani, and Dwinita W. Utami. Indonesia<br />possesses a great number of sweet potato varieties.<br />Understanding the diversity and distribution of this genetic<br />resource is essential for its management and future use. The<br />objective of this study was to elaborate the molecular<br />character as DNA finger print of Indonesian sweet potato<br />germplasm. Eight fluorescent labeled SSR primers were<br />used to amplify DNA of 88 sweet potato accessions<br />consisting of improved varieties and landraces collected<br />from 7 islands in Indonesia. The amplified products were<br />detected using capillary electrophoresis method in CEQ<br />Genetic Analysis System machine. A total of 135 alleles<br />ranging from 8 to 36 alleles per locus with an average of 17<br />alleles were generated. Each accession had a unique<br />microsatellite finger print marked by specific combination of<br />11 to 22 alleles in 8 SSR loci. Dendrogram generated by<br />UPGMA based on simple matching coefficients produced 4<br />nonspecific groups at 80% similarity. The groups revealed<br />the possibilities that the accessions were distributed from<br />similar genetic resources.</p>