Abstract
Confluent monolayers of cultured human endothelial cells were exposed to suspensions of normal and sickle erythrocytes. The normal RBC were found to be nonadherent to endothelium. However, sickle RBC adhered rather tenaciously to endothelial cells, a behavior that required neither deoxygenation nor morphologic distortion of the RBC. Studies on manipulated RBC indicated that the presence of RBC membrane sialic acid is necessary for endothelial adherence to occur. Although sickle RBC were found to have normal amounts of sialic acid, electron microscopic localization of these residues by means of cationized ferritin labelling indicated that sialic acid is distributed abnormally on the sickle RBC surface. Further study of manipulated normal RBC implicated an abnormal accumulation of calcium (a property of sickle RBC) in the development of both propensity for endothelial adherence and abnormal sialic acid distribution observed for sickle RBC.
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