Abstract
We have investigated glycoconjugates sialization profile, endogen synthesis rate of antiganglioside antibodies (AGA), IL-6 signaling pathways correlated with activity disease in systemic lupus erythematous (SLE) and lupus nephritis (LN). Material and methods. A case-control study was developed and included 109 patients with SLE with or without renal impairment, 32 patients with IgA nephropathy and 60 healthy volunteers, clinically and paraclinically monitored. The following parameters were evaluated in volunteers serum: total sialic acid (TSA), orosomucoids, lipid bound sialic acid (LSA), interleukin-6 (IL-6), soluble factors IL-6R, gp130, anti –GM1, -GM2, -GM3, -GD1a, -GD1b, -GT1b, -GQ1b antigangliosides antibodies of IgG and IgM type. Results. Experimental data analysis showed: increase in synthesis rhythm of sialoglyco-conjugated in SLE (TSA increased in SLE and LN compared to control), accelerated catabolism of LSA in LN (LSA/TSA ratio was higher in SLE and LN than in control group), overexpression of IL-6 mediated trans-signaling (sIL-6R/sgp 130 ratio was subunit in SLE and IgA nephropathy and superunit in LN), large AGA profile synthesis of IgM isotype (over 45.1% in SLE and over 20.7% in LN). Conclusions. Hypersialization, accelerated glycosphingolipids degradation, IL-6 trans-signaling amplify and AGA pattern could represent essential mechanisms in LN pathogenesis.
Highlights
Inflammation was assessed by C reactive protein, and we found high inflammation in systemic lupus erythematous (SLE) and lupus nephritis (LN) groups compared with control (p < 0.05) and in LN compared with SLE group (p < 0.05)
The results of the present study showed that SLE is associated with glycoproteins and glycolipids metabolism alteration
lipid bound sialic acid (LSA)/total sialic acid (TSA) ratio could be considered a molecular target for early evaluation of LN
Summary
N-acethyl-neuraminic acid (acid sialic, Neu5Ac, NANA) is a negatively charged molecule, found as terminal monozaharide in glyco-conjugates, essential metabolite in human physiology and pathology. Syalyl-conjugates are involved in cells interaction, cellular aggregation, intercell communication and signaling, development of immunity and renal function. Circulant glycoproteins (orosomucoids or alpha-1-acid glycoprotein) have important functions in immune and inflammatory response and could play a primary role in SLE disease activity and relapse [11,12]. Glycosphingolipids are involved in autoimmune diseases pathogenesis and renal disfunctions [13,14,15]. These molecules were little investigated in lupus nephritis (LN) during years [10]
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