Abstract
Colorectal and glioblastoma cancer stem‐like cells (CSCs) are essential for translational research. Cell line authentication by short tandem repeat (STR) profiling ensures reproducibility of results in oncology research. This technique enables to identify mislabeling or cross‐contamination of cell lines. In our study, we provide a reference dataset for a panel of colorectal and glioblastoma CSCs that allows authentication. Each cell line was entered into the cell Line Integrated Molecular Authentication database 2.1 to be compared to the STR profiles of 4485 tumor cell lines. This article also provides clinical data of patients from whom CSCs arose and data on the parent tumor stage and mutations. STR profiles and information of our CSCs are also available in the Cellosaurus database (ExPASy) as identified by unique research resource identifier codes.
Highlights
Cancer research requires models that use patient-derived cultured cells
We characterized 18 colorectal cancer stem-like cell (CSC) lines (CTSCs)[11] from 17 patients and 103 glioblastoma CSCs (GSCs)[3] from 95 patients by short tandem repeat (STR) assay to create a reference dataset that allows the determination of the authenticity of these cell lines and ensures the reliability and reproducibility of research experiments
In two glioblastoma patients, of whom we obtained GSC lines from both primary surgery and surgery for recurrence, the STR profile confirmed the authenticity of CSC lines derived from the same patient
Summary
Cancer research requires models that use patient-derived cultured cells. These models allow to study tumor heterogeneity, in particular in the early stages of tumorigenesis. We characterized 18 colorectal CSC lines (CTSCs)[11] from 17 patients and 103 glioblastoma CSCs (GSCs)[3] from 95 patients by STR assay to create a reference dataset that allows the determination of the authenticity of these cell lines and ensures the reliability and reproducibility of research experiments. In two glioblastoma patients, of whom we obtained GSC lines from both primary surgery and surgery for recurrence, the STR profile confirmed the authenticity of CSC lines derived from the same patient. Duplicates were found only in those cell lines that derived from different regions of the same tumor
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