Short-chain fatty acids present in the ileum inhibit fasting gastrointestinal motility in conscious pigs.

Abstract

Colonic compounds, primarily short-chain fatty acids (SCFAs), inhibit gastric tone in humans. However, since colonic compounds reflux into the ileum, SCFAs might act also at the ileal level. This study evaluates the effects of the contact of SCFAs with the ileum alone towards gastrointestinal motility in fasted conscious pigs. Gastrointestinal motility was recorded during ileal infusion of a mixture containing acetate, propionate and butyrate in intact vs ileostomized animals (n = 10). Ileal infusions of isomolar (500 mmol L-1) vs isocaloric (600 kJ L-1) SCFAs were also performed. SCFA mixture reduced significantly the amplitude and increased the frequency of antral contractions in intact (motility index: 2624 +/- 503.4 vs 4077 +/- 388.2) and ileostomized pigs (motility index: 2428 +/- 678.1 vs 4709 +/- 773.4) compared with iso-osmotic saline. SCFAs at isomolar concentrations inhibited equally gastric motor pattern irrespective of their chemical structure. On the contrary, isocaloric concentrations of SCFAs induced graded effects: acetic acid being the most potent to reduce gastric motility. In conclusion, SCFAs inhibit gastrointestinal motility by a direct contact with the ileum. This inhibition was concentration dependent.