Shoot regeneration from nodules of Charybdis sp.: A comparison of semisolid, liquid and temporary immersion culture systems

Abstract

Nodules of Charybdis numidica maintained in liquid Murashige and Skoog (MS) medium with 20 µmol BA in the dark were subjected to different treatments under continuous light for shoot regeneration. A high regeneration rate without hyperhydration of the shoots was observed on semisolid basal MS medium with 1 % sucrose. The use of liquid MS medium (1 % sucrose, no growth regulators) resulted in a significantly lower amount of shoots per gramme of nodules under both submerged and temporary immersion (TI) conditions. Shoot hyperhydration was lowest in a TI system with one 5 min immersion every 24 hours. When compared on a per container base, large amounts of shoots could be produced in the TI system with less labour input than in the system with semisolid medium.