Abstract
1. 1. Five isozymic forms of lactate dehydrogenase ( l-lactate: DPN oxidoreductase, EC 1.1.1.27) have been observed in rat epididymal adipose tissue. In untreated animals the distribution of lactate dehydrogenase activity among the five isozymes was such that LDH I (H 4) (electrophoretically the most anodal) was present in the least activity with the activity in the isozyme spectrum increasing toward LDHV (M 4). 2. 2. Upon fasting for periods of 48–120 h, the distribution of lactate dehydrogenase in the adipose tissue shifted toward LDH I. This increase in LDH I was due to a significant increase in the percentage of H subunits in the fasted adipose tissue. 3. 3. Refeeding fasted rats for varying periods of time resulted in a gradual return of the percentage of H subunits to the control level. Complete recovery was seen only in those animals which had been refed for a period of time equal to that of the fasting period. 4. 4. Treatment of fasting rats with actinomycin D, puromycin, ethionine, or methionine resulted in inhibiting or abolishing the relative increase in the H subunits that occurs during fasting.
Published Version
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