Shape variability and morphological discrimination of haptoral attachment structures in Urocleidoides spp. Mizelle et Price, 1964 (Monogenea: Dactylogyridae) parasitizing fish from neotropical rivers in southern Mexico

Abstract

The morphology of attachment organs in Monogenea is an essential source of evidence for taxonomic assessments and understanding of ecology and evolutionary history. It has been demonstrated that geometric morphometry analysis of the haptoral attachment organs provides rich and precise information about monogeneans morphology. In this study, we consider three monogenean species of Urocleidoides, U. simonae, U. vaginoclaustrum, U. vaginoclaustroides, and the incertae sedis species U. strombicirrus that parasitize fish of the Poeciliidae, Profundulidae and Characidae in neotropical rivers in southern Mexico. We aimed to identify new morphological characteristics that can be used to describe these populations with greater certainty and to test whether these characteristics present geographic variation. Anchor shape data (n = 69) were generated using geometric morphometric methods. We discriminated species using principal components and canonical variate analysis of shape data. Our results showed that there was discernible interspecific variation. The four species contributed four new morphological characteristics: anchor point length, short versus long anchor roots, elongated versus wide anchor shape, and the relative length of inner versus outer roots. Intraspecific variation was relatively low compared to interspecific anchor shape variation in Urocleidoides spp. These new morphological characteristics may also be useful for delimiting other similar species in Monogenea.