Shape of Pods of Medicago Truncatula as a Promising Morphological Marker for Embryogenic Capacity

Abstract

ABSTRACTThree different types of pods (N- normal with spines, LS- less spines and WS-without spines) were harvested from Medicago truncatula cv. R 108 1 plants grown in greenhouse. The pods with changed shape LS and WS were developed at the end of period of plant maturity at the upper part of plants. In vitro plants originated from seeds from three types of pods were obtained. Callus tissue produced from leaves and petioles of plants from type N, LS and WS showed different embryogenic potential. Callus tissue of type N formed embryos from both -leaves and petioles, while the callus from type WS was able to form embryos only from petioles. Regardless of the initial explants callus from LS plants did not show any embryogenic ability. In contrast to the regenerants from N type capable to form normal root system, those from WS type possess poor developed root system.