Abstract
Genetic mutant resources are very useful and necessary tools to elucidate functions of the very large number of genes present in plant genomes. Here, we report the large mutant resources that we have developed in the model legume, Medicago truncatula cv. Jemalong A17, using a physical mutagen, fast neutron. We first discuss the scale of the mutant resources with an aim of saturation mutagenesis. We then discuss methods that have been used for high-throughput molecular cloning of underlying genes in fast neutron bombardment (FNB)-induced mutants in M. truncatula. And lastly, we briefly summarize some FNB mutants that have been studied to facilitate our understanding of the molecular mechanisms that underlie a broad range of developmental processes such as compound leaf and seed development, root gravitropism, and symbiotic nitrogen fixation (SNF) in legumes.Keywords Medicago truncatula LegumesFast neutron bombardment mutantsMolecular cloningSymbiotic nitrogen fixation
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