Sexual Activity and Plasma Levels of Sex Steroids in the Aspic Viper Vipera aspis L. (Reptilia, Viperidae)

Abstract

Reproductive behavior and associated sexual activity was studied in individual male and female Vipera aspis over a 3-year period in western France in an attempt to correlate mating behavior with blood levels of gonadal sex steroids. Males had higher average levels of both testosterone (T) and 5α-dihydrotestosterone (DHT) than females. Levels of progesterone (P) did not differ significantly between the two sexes but estradiol-17β (E 2) concentrations were significantly higher in females during the season of mating. Spring mating behavior and copulation in males was associated with significantly increased levels of T and DHT, compared with postmating males; and a similar, but not significant trend, was evident with autumnal mating. The only statistically significant hormonal difference detected in males showing no sexual activity in autumn, was an elevated level of E 2 at 0.52 ± 0.20 ng/ml compared with 0.09 ± 0.03 ng/ml in spring-breeding males ( P = 0.05). Estrus in females is associated with increased levels of all four steroids but significant only for E 2 and DHT. Levels of P were significantly reduced in females displaying seasonal anestrous in the spring immediately following reproduction. Females not displaying estrus in either spring or autumn had significantly lower plasma DHT and E 2. Although mating behavior in males is associated statistically with elevated levels of T and DHT, a tight correlation is not obvious at the individual level, suggesting that increased concentrations of androgens are a necessary, but not sufficient, condition for mating. The data from V. aspis suggest that, as in a number of other reptilian species, high circulating levels of androgens function to "condition" or "organize" sexual behavior in males which may be displayed at some later time, well after actual levels have fallen, thus engendering the impression that reproductive behavior may be temporally dissociated from essential hormonal stimuli.