Abstract
Mungbean yellow mosaic virus-[India:Vigna] (MYMV-[IN:Vig]), a blackgram isolate of MYMV, has five variable and infective DNA B components of which KA22 and KA27 DNA Bs share only 72% nucleotide sequence identity between them. Agroinoculation of blackgram with partial dimers of DNA A and KA27 DNA B caused severe stunting and an inordinate delay in flowering. Interestingly, co-agroinoculation of KA27+KA22 DNA B components along with DNA A ameliorated severe stunting, rescued from the delay in flowering and caused the appearance of yellow mosaic symptom characteristic of KA22 DNA B. Post-agroinoculation of KA27 DNA B-infected blackgram plants with KA22 DNA B also resulted in the amelioration from severe stunting and in the alleviation from the delay in flowering. Alleviation from KA27 DNA B-type of symptom by co-infection or post-infection with KA22 DNA B did not result in a corresponding reduction in KA27 DNA B levels. Swapping of KA27 DNA B with the nuclear shuttle protein gene (NSP) of KA22 DNA B abolished severe stunting and caused the appearance of mild yellow symptom, suggesting that the NSP is the major symptom determinant in MYMV DNA B.
Published Version
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