Abstract
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Highlights
9–52 ND ND 333 347 178 128 94IU: international unit; ND: not done. a The sampling day refers to the number of days after symptom onset
Five zoonotic hantaviruses have been described in Europe: PUUV, Dobrava-Belgrade (DOBV), Tula (TULV), Saaremaa (SAAV), and SEOV
To the best of our knowledge, that is the first report of SEOV infection in a pregnant woman, we suppose such cases of infection may have been suspected or detected in Asia [13,14]
Summary
IU: international unit; ND: not done. a The sampling day refers to the number of days after symptom onset. For surveillance purposes, the positive sample was transferred to the National Reference Centre for Hantavirus, where these positive results were confirmed by an enzyme-linked immunosorbent assay (ELISA) developed in-house using Hantaan virus (HTNV) and Puumala virus (PUUV) antigens [1]. The complete S coding domain sequence was recovered via three nested RT-PCRs with in-housedesigned primers (protocol available on request from the authors), producing three overlapping amplicons (GenBank accession number KC902522). The amino acid sequence was identical to those of SEOV strains found in the United Kingdom, Vietnam, South Korea and China (GenBank accession numbers JX879769, AB618112, NC_005236 and GU592947, respectively). It was identical to the partial S coding domain sequence obtained from the SEOV strain detected in rodents in France [5]. Using MEGA version 5.1 [6], phylogenetic analysis based on S coding domain sequence confirmed that the strain – named REPLONGES/Hu/ FRA/2012/12-0882 – belonged to SEOV species, especially to the main phylogroup, which includes strains from Asia and the rest of the world [7] (Figure)
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