Serum thyroid hormone concentrations and thyroid histomorphology as biomarkers in bowhead whales (Balaena mysticetus)

Abstract

Serum thyroid hormone (TH) concentrations have been used alone or with other measurements to assess health status or effects of toxicant exposure in marine mammals. Histological sections from thyroid glands of the bowhead whale ( Balaena mysticetus L., 1758) were examined in conjunction with serological TH analyses. Serum was assayed for total and free triiodothyronine and total and free thyroxine via radioimmunoassay. Histomorphology of thyroid tissue was assessed by light microscopy and the utilization of an epithelial-follicular index (EFI). Age, sex, or season did not significantly affect serum TH levels. However, TH concentrations in pregnant or lactating females were found to be significantly lower than in the other sex and reproductive groups investigated. The EFI and epithelial height (EH) were greater in spring subadult and adult whales compared with those that were landed in the fall. No correlation was found between serum TH concentrations and serum, blubber, or liver levels of select polychlorinated biphenyl metabolites and organochlorine congeners examined. Low variability in concentrations of the serum THs across age, season, and sex and reproductive groups supports the existence of strong homeostatic mechanisms for maintaining TH concentrations in these presumably healthy animals. Departures from these ranges may indicate a disturbance in these regulatory mechanisms and may be a useful indication of toxicity or other health disorders.